A highly stable sucrose polymer with an average molecular weight of 400 000, suitable for isolating eukaryotic cells and organelles with minimal damage.
For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
Better osmotic properties than sucrose.
Preserves functional and morphological integrity.
Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
Does not penetrate biological membranes.
Serves as the raw material for preparation of Ficoll-Paque gradients.
Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
High density without increased osmotic pressure
Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose with an average molecular weight of 400 000. It has long been used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilizing agent, and as a preparation medium for isolating mononuclear cells.
Applications can also be found in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies.
Highly soluble
High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media. READ LESS
Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State
Solid
Physical Form
Spray-dried powder
Molecular Weight (Mr) Max.
~ 5 ¡¿ 10~ 5
Molecular Weight (Mr) Min.
~ 3 ¡¿ 105
Reactivity and Stability
The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110¡ÆC for 30 min without degradation. Strong oxidizing and reducing agents should be avoided
Stokes Radius
~ 10 nm
Color
White
Specific Rotation (alpha)20
50¡Æ-65¡Æ
Relative Viscosity
5 ¥çr at 20¡ÆC: 10% (w/v) 20 ¥çr at 20¡ÆC: 20% (w/v) 60 ¥çr at 20¡ÆC : 30% (w/v 180 ¥çr at 20¡ÆC : 40% (w/v) 600 ¥çr at 20¡ÆC : 50% (w/v)
Intrinsic Viscosity
~ 17 ml/g
Solubility
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained
Certificate of Analysis
Yes
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Ficoll PM400 (Dry Powder)
Parameter
Ficoll PM400 (Dry Powder)
Application
Cell isolation
Density Max.
1.2 g/ml
Endotoxin Activity Max.
0.12 EU/mg
Estimated Shelf Life from Manufacture Date
5 years
Storage conditions
4–30¡ÆC
Stability
Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State
Solid
Physical Form
Spray-dried powder
Molecular Weight (Mr) Max.
~ 5 ¡¿ 10~ 5
Molecular Weight (Mr) Min.
~ 3 ¡¿ 105
Reactivity and Stability
The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110¡ÆC for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.
Stokes Radius
~ 10 nm
Color
White
Specific Rotation (alpha)20
50¡Æ-65¡Æ
Relative Viscosity
5 ¥çr at 20¡ÆC: 10% (w/v) 20 ¥çr at 20¡ÆC: 20% (w/v) 60 ¥çr at 20¡ÆC : 30% (w/v 180 ¥çr at 20¡ÆC : 40% (w/v) 600 ¥çr at 20¡ÆC : 50% (w/v)
Intrinsic Viscosity
~ 17 ml/g
Solubility
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained