Ready-to-use sterile density gradient media designed for the preparation of mononuclear cells from peripheral blood, bone marrow and cord blood.
Manufactured under a Quality Management System certified to ISO 13485:2012.
Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
Cytotoxicity grade 0.
Choice of densities: 1.077, 1.084, and 1.073 g/mL.
A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
Ficoll-Paque PREMIUM vs Ficoll-Paque PLUS
All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2012
How it's used
Ficoll-Paque PREMIUM: Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Ficoll-Paque PREMIUM 1.084: Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Ficoll-Paque PREMIUM 1.073: Can be used when isolating lower-density human mononuclear cells (e.g. mesenchymal stromal cells or monocytes).
Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Endotoxin Activity Max.
< 0.12 EU/ml
Properties
Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated Shelf Life from Manufacture Date
3 years
Storage conditions
4–30¡ÆC
Stability
Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4¡ÆC and 30¡ÆC and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution
Physical State
Liquid
Osmolality
288-310 mOsm/kg
pH Range
5.5–7.5
Color
Colorless to slightly yellow
Certificate of Analysis
Yes
SELECT YOUR MODEL
Ficoll-Paque PREMIUM 1.073
Parameter
Ficoll-Paque PREMIUM 1.073
Application
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
Endotoxin Activity Max.
< 0.12 EU/ml
Properties
Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated Shelf Life from Manufacture Date
3 years
Storage conditions
4–30¡ÆC
Stability
Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4¡ÆC and 30¡ÆC and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution
Physical State
Liquid
Osmolality
276-298 mOsm/kg
pH Range
5.5–7.5
Color
Colorless to slightly yellow
Certificate of Analysis
Yes
SELECT YOUR MODEL
Ficoll-Paque PREMIUM 1.084
Parameter
Ficoll-Paque PREMIUM 1.084
Application
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Endotoxin Activity Max.
< 0.12 EU/ml
Properties
Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated Shelf Life from Manufacture Date
3 years
Storage conditions
4–30¡ÆC
Stability
Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4¡ÆC and 30¡ÆC and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution