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FrameStar¢ç 384 Well Skirted PCR Plate
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PCR
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FrameStar¢ç 384 Well Skirted PCR Plate
Polypropylene wells, polycarbonate frame, cut corner A24, working volume: <30 µl, total well capacity: 55 µl; designed for use on standard thermal cyclers
Available in the following colours
"FrameStar¢ç plates led to significantly better results and reduced evaporation compared to standard PCR plates."
Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany
Product Information
Polypropylene wells, polycarbonate frame, cut corner A24, working volume: <30 µl, total well capacity: 55 µl; designed for use on standard thermal cyclers
Our FrameStar¢ç 384 Well Skirted PCR Plates are designed for high-throughput PCR. They are compatible with the majority of 384 well block PCR, qPCR and sequencing instruments, and the rigid 2-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.
The skirt allows for labelling or barcoding.
Technical Specifications
Plate Width |
127.76 ¡¾ 0.25 mm |
Plate Depth |
85.48 ¡¾ 0.25 mm |
Plate Height |
10.60 ¡¾ 0.25 mm |
Well Depth |
9.60 ¡¾ 0.10 mm |
Well Diameter |
3.10 ¡¾ 0.10 mm |
Distance to centre of A1 from top edge |
8.99 ¡¾ 0.25 mm |
Distance to centre of A1 from left edge |
12.13 ¡¾ 0.25 mm |
Pitch (distance between A1 and A2) |
4.50 mm |
Plate Features
- Recommended for low volume PCR
- Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- Improved seal integrity due to thermal stability of the frame
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
- <30 µl working volume, 55 µl total well capacity
- Compatible with the majority of 384 well block PCR, qPCR and sequencing instruments
- Alphanumeric grid reference to aid well and sample identification
- 6 frame colour options for the clear well variety - purple, blue, clear, green, red, and black
- Also available as a black frame with white wells (for optimum signal-to-noise ratio when using fluorescent based assays); as a black frame with black wells (for minimal light diffusion and interference); and as a clear frame with frosted wells (see below)
Now Available - Frosted Wells for use with ABI¢ç/Life Technologies¢ç qPCR Instruments
While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.
However, white well PCR plates may lead to software related problems with ABI¢ç/Life Technologies¢ç cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.
In response to this, we at 4titude¢ç have developed a frosted well variety of the FrameStar¢ç 384 well plate to offer ABI¢ç/Life Technologies¢ç instrument users the time and money-saving advantages of FrameStar¢ç. And as the signal levels are directly comparable with ABI¢ç/Life Technologies¢ç plates, there's no need for users to recalibrate their instruments.
Find these plates in the Order tab under product code 4ti-0387.
Frosted wells compared to clear wells
Barcoding
This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
Compatibility
MANUFACTURER |
BLOCK TYPE |
INSTRUMENT |
ABI¢ç / LIFE TECHNOLOGIES / THERMO FISHER SCIENTIFIC |
Thermal Cyclers (384 well block) |
GeneAmp¢ç 9700 | Verity | Proflex | Multiblock system |
qPCR Cyclers (384 well block) |
QuantStudio¢â 5 / 6 / 7 / 12K | ViiA7¢â | 7900 HT FAST |
Sequencers (384 well block) |
3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL |
AGILENT / STRATAGENE |
Thermal Cyclers (384 well block) |
SureCycler 8800 |
ANALYTIK JENA / BIOMETRA |
Thermal Cyclers (384 well block) |
Flexcycler2 | T1 Thermocycler | Tadvanced | TProfessional | Trobot 96 |
qPCR Cyclers (384 well block) |
qTOWER3 84 / 84G |
BIOER TECHNOLOGIES |
Thermal Cyclers (384 well block) |
Gene Touch 384 |
BIO-RAD |
Thermal Cyclers (384 well block) |
C1000 Touch | S1000 |
qPCR Cyclers (384 well block) |
CFX384 Touch |
BIO-RAD MJ RESEARCH |
Thermal Cyclers (384 well block) |
PCT200¢â / 220¢â / 221¢â / 225¢â / 240¢â |
CORBETT RESEARCH |
Thermal Cyclers (384 well block) |
(Qiagen) Palm Cycler 384 |
EPPENDORF |
Thermal Cyclers (384 well block) |
MasterCycler¢ç ep 384 / Pro 384 |
GE HEALTHCARE / AMERSHAM |
Sequencers (384 well block) |
MegaBACE¢â 4000 |
PEQLAB / VWR |
Thermal Cyclers (384 well block) |
peqSTAR 384X |
SENSOQUEST |
Thermal Cyclers (384 well block) |
Labcycler |
TECHNE |
Thermal Cyclers (384 well block) |
Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1 / 2/ 4 | TC412 | TC512 | Genius | Genius Quad | Flexigene |
For recommended plate options please refer to the Instrument Compatibility Table.
Ordering
FrameStar¢ç 384 Well Skirted PCR PlatePolypropylene wells, polycarbonate frame, cut corner A24, working volume: <30 µl, total well capacity: 55 µl; designed for use on standard thermal cyclers |
Code |
Description |
Plates/Case |
4ti-0384 |
clear wells, purple frame |
50 plates |
4ti-0384/B |
clear wells, blue frame |
50 plates |
4ti-0384/C |
clear wells, clear frame |
50 plates |
4ti-0384/G |
clear wells, green frame |
50 plates |
4ti-0384/R |
clear wells, red frame |
50 plates |
4ti-0384/X |
clear wells, black frame |
50 plates |
4ti-0385 |
white wells, black frame |
50 plates |
4ti-0386 |
black wells, black frame |
50 plates |
4ti-0387 |
frosted wells, clear frame |
50 plates |
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