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FrameStar¢ç 96 Well Skirted Optical Bottom PCR Plate
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PCR
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FrameStar¢ç 96 Well Skirted Optical Bottom PCR Plate
Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
Available in the following colours
"FrameStar¢ç plates led to significantly better results and reduced evaporation compared to standard PCR plates."
Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany
Product Information
Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
We have now extended our FrameStar¢ç range to include PCR plates with optically clear flat bottom wells for microscopy applications.
The FrameStar¢ç 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Random Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR. Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing.
The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.
Technical Specifications
Plate Width |
127.76 ¡¾ 0.25 mm |
Plate Depth |
85.48 ¡¾ 0.25 mm |
Plate Height |
16.10 ¡¾ 0.25 mm |
Well Depth |
12 ¡¾ 0.10 mm |
Well Diameter |
5.46 ¡¾ 0.10 mm |
Distance to centre of A1 from top edge |
11.24 ¡¾ 0.25 mm |
Distance to centre of A1 from left edge |
14.38 ¡¾ 0.25 mm |
Pitch (distance between A1 and A2) |
9.00 mm |
The low profile wells of this plate are shorter than "standard" profile wells, which decreases the "dead space" between the heated lid of the thermal cycler and the reagents in the well. This reduces condensation forming on the side wall of the tubes, moderating changes in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.
Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes. The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes.
The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting.
Figure 1. Technical drawing of a conical PCR tube (A) compared to a flat bottom PCR tube (B)
Plate Features
- Suitable for microscopy and small volume sample storage
- Recommended for low volume PCR
- Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- Improved seal integrity due to thermal stability of frame
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
- <0.1 ml (100 µl) working volume, 0.18 ml (180 µl) total well capacity
- Alphanumeric grid reference to aid well and sample identification
Barcoding
This plate is available with linear Code 128-format barcodes to aid identification and traceability of your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
Compatibility
MANUFACTURER |
BLOCK TYPE |
INSTRUMENT |
AGILENT / STRATAGENE |
Thermal Cyclers (96 well block) |
Robocycler Gradient |
qPCR Cyclers (96 well block) |
AriaMx |
ANALYTIK JENA / BIOMETRA |
Thermal Cyclers (96 well block) |
Flexcycler2 | T1 Thermocycler | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard / Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR) |
BIO-RAD |
Thermal Cyclers (96 well block) |
C1000 Touch | S1000 |
qPCR Cyclers (96 well block) |
CFX96 Touch | CFX 96 Touch Deep Well | CFX connect |
BIO-RAD MJ RESEARCH |
Thermal Cyclers (96 well block) |
PCT100¢â / 200¢â / 220¢â / 221¢â / 225¢â / 240¢â |
qPCR Cyclers (96 well block) |
Chromo4¢â | Opticon2¢â |
EPPENDORF |
Thermal Cyclers (96 well block) |
MasterCycler¢ç ep / ep gradient / Pro / Pro S / nexus / nexus gradient / nexus SX1 / nexus GSX1 |
qPCR Cyclers (96 well block) |
MasterCycler¢â ep realplex |
GE HEALTHCARE / AMERSHAM |
Sequencers (96 well block) |
MegaBACE¢â 500 | MegaBACE¢â 1000 mark 2 |
PEQLAB / VWR |
Thermal Cyclers (96 well block) |
peqSTAR 96X |
SENSOQUEST |
Thermal Cyclers (96 well block) |
Labcycler |
TECHNE |
Thermal Cyclers (96 well block) |
TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene |
qPCR Cyclers (96 well block) |
Quantica |
For recommended plate options please see the Instrument Compatibility Table and reference the FrameStar¢ç 96 Well Skirted PCR Plate (4ti-0960).
Ordering
FrameStar¢ç 96 Well Skirted Optical Bottom PCR PlateLow profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl |
Code |
Description |
Plates/Case |
4ti-0970 |
clear wells, clear frame |
50 plates |
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