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FrameStar¢ç 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
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PCR
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FrameStar¢ç 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler¢ç 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
Available in the following colours
"FrameStar¢ç plates led to significantly better results and reduced evaporation compared to standard PCR plates."
Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany
Product Information
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler¢ç 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
Our FrameStar¢ç Roche Style plates are designed to achieve optimised assay conditions on the Roche LightCycler¢ç 96 and 480 (with 96 well block).
This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.
The extra white wells allow for increased sensitivity in fluorescent assays.
The semi-skirted frame allows for labelling or barcoding.
Technical Specifications
Plate Width |
127.70 ¡¾ 0.25 mm |
Plate Depth |
85.48 ¡¾ 0.25 mm |
Plate Height |
15.60 ¡¾ 0.25 mm |
Well Depth |
15.10 ¡¾ 0.10 mm |
Well Diameter |
5.50 ¡¾ 0.10 mm |
Distance to centre of A1 from top edge |
11.24 ¡¾ 0.25 mm |
Distance to centre of A1 from left edge |
14.38 ¡¾ 0.25 mm |
Pitch (distance between A1 and A2) |
9.00 mm |
Plate Features
- Compatible with the Roche LightCycler¢ç 96 and 480 (with 96 well block)
- Recommended for low volume PCR
- Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- Improved seal integrity due to thermal stability of frame
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
- <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
- Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
- Alphanumeric grid reference to aid well and sample identification
Application Data
Ct values of data run on a Roche LightCycler¢ç 480, comparing 4titude FrameStar¢çRoche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates. The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct values.
Barcoding
This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
Compatibility
MANUFACTURER |
BLOCK TYPE |
INSTRUMENT |
ROCHE |
qPCR Cyclers (96 well block) |
LC96 | LC480 |
For recommended plate options please refer to the Instrument Compatibility Table.
Ordering
FrameStar¢ç 96 Well Semi-Skirted PCR Plate, Roche Style, High SensitivityLow profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler¢ç 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays |
Code |
Description |
Plates/Case |
4ti-0954 |
extra white wells, clear frame |
50 plates |
Combi Pack Code |
Description |
Plates & Seals/Case |
4ti-0954/0560 |
4ti-0954 + qPCR Seal (4ti-0560) |
50 plates + 50 seals |
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